The combined effect of drought and light on different physiological and biochemical traits was assessed in cork oak (Quercus suber L.) seedlings grown under two levels of light availability and submitted to a long-standing drought. Watering was withdrawn after germination and seedlings were allowed to dry to a water content of ca. 50% of field capacity. At this point, water-stressed seedlings were grown under moderate drought and two light regimes: high light (HL—50%) and low light (LL—2%). Soil water in control plants was kept close to field capacity (90–100%) for both light environments. Water-relations parameters derived from P–V curves, gas exchange and water status at predawn (Ψpd) were evaluated at twice during the experiment. Nitrogen and chlorophyll contents were determined in the same leaves used for the gas exchange measurements. In addition, maximum rate of carboxylation (Vcmax) and electronic transport (Jmax) were derived from A–Ci curves in well-watered seedlings.
The variation on moisture availability during the experiment was the same under both light environments. In control plants, Ψpd was over −0.3 MPa at the two harvests, while stressed seedlings decreased to −0.9 MPa, with no differences between light treatments. Water stress decreased osmotic potentials at full (Ψπ100) and zero turgor (Ψπ0). The regressions between both potentials and Ψpd showed a higher intercept in shade grown seedlings. This fact will point out the higher osmoregulation capacity in sun seedlings whatever water availability.
Nitrogen investment on a per leaf mass (Nmass), chlorophyll content (Chlmass) and SLA tended to show a typical pattern of sun-shade acclimation. Thus, the three parameters increased with shade. Only for Nmass there was a significant effect of watering, since water stress increased Nmass.
LL plants showed a lower photosynthetic capacity in terms of maximum net photosynthesis at saturating light (Amax), which was related to a decrease in Vcmax and Jmax. Both parameters varied with specific leaf area (SLA) in a similar way. The low-light environment brought about a higher nitrogen investment in chlorophyll, while under high-light environment the investment was higher in carboxylation (Vcmax) and electronic transport (Fmax).
Stomatal conductance to water vapour (gwv) and Amax were lower in low-light seedlings independently of watering. In addition, there was a trend to keep higher intrinsic water use efficiency (IWUE) under high light environment. The increase of IWUE under water stress was higher in HL seedlings. This was as consequence of the steeper decline in gwv as Ψpd decreased. The decrease of Amax with Ψpd occurred in a similar way in LL and HL seedlings. Thus, the HL seedlings tended to sustain a higher ability to increase IWUE than LL seedlings when they were submitted to the same water stress. 相似文献
The study was carried out in a 9-year-old hinoki cypress (Chamaecyparis obtusa (Sieb. et Zucc.) Endl.), stand over a span of three years from July 1992 to June 1995, primarily to predict litter production
from exteral tree dimensions by combining open-top clothtrap and clipping methods. Litter production was virtually concentrated
in October and November. Stem cross-sectional area at the crown base was proved to be the reliable predictor of litter production,
and that single regression model was evolved irrespective of year. The regression model had proportional constants of 2.696
× 10−2 and 3.540 × 10−2 kg cm−2 year−1 for leaf litter and total litter production, respectively. Utilizing the model, leaf litter production of the stand was assessed
to be 5.04, 5.12, and 4.99, and total litter production to be 6.48, 6.58, and 6.40 Mg ha−1 year−1 for the first, second and third year, respectively. Biomass increment was 6.67, 7.80, and 7.70, tree mortality was 0.15,
0.13, and 0.41, and insect grazing was 0.09, 0.05, and 0.002 Mg ha−1 year−1 for the first, second and third year, respectively. Above-groud net production was therefore 13.39, 14.55, and 14.51, Mg
ha−1 year−1, and biomass accumulation ratio (biomass/net production) was 1.86, 2.21, and 2.76 year for the first, second and third year,
respectively. Considering data from earlier studies and the results of this study, biomass accumulation ratio,BAR (year), of hinoki stands was best related to above-ground biomass,y (Mg ha−1), using the power function:BAR=0.112y0.936. Excluding seedling stands, leaf efficiency (above-ground net production per unit leaf mass) of hinoki stands was 0.91±0.02
(SE) Mg Mg−1 year−1, irrespective of stand biomass or age. 相似文献
The present study was carried out to elucidate the drought growth responses of Quercus ilex L., Phillyrea latifolia L., Arbutus unedo L., and other accompanying woody species of the Mediterranean holm oak forest. We submitted holm oak forest stands in Prades mountains (NE Spain) to a 2-year experimental drought. We reduced soil water availability about 15% by plastic strips and funnels that partially excluded rain throughfall and by ditch interception of water runoff. Mean stem diameter increment showed a great variation depending on the species. A. unedo had larger growth rates than Q. ilex and P. latifolia, but it was also the species that experimented the highest growth reduction in the drought plots (77%), suggesting a higher drought sensitivity than Q. ilex (55%) and P. latifolia (no drought effect). The growth reduction was specially marked in the larger trees. Aboveground stand biomass increment, estimated from stem diameter by allometric relationships, was 1.9 Mg ha−1 per year in the control plots. The 15% reduction in the upper soil moisture produced 42% reduction in this biomass increment. In the drier conditions predicted in this Mediterranean area in the frame of climate change, an important reduction of growth rates can be hence expected, accompanied by a gain of dominance of drought-tolerant species such as P. latifolia in detriment of more mesic species such as Q. ilex. 相似文献
The studies on leave protein of Betula platyphylla seedlings by the methods of SDS-PAGE electrophoresis and dimensional gel electrophoresis was concerned. The result indicated that some new proteins such as 29kD pI4.3, 29kD pI4.2, 14kD pI5.3 and 11.5kD pI5.16 protein point were induced by soil drought stress, and some protein disappeared,for instance four protein between 12.4 - 11.5kD pI5.8 - 6.44 in seedlings leaves of B. platyphylla during soil drought stress. 相似文献